WebPMA induced expression of RPTPb mRNA and protein as determined by RNase protection assay and indirect immunofluorescence studies, respectively. Vitamin D 3 and interferon-g, which stimulate differentiation of HL-60 cells into monocyte-like cells, also induced VacA sensitivity and expression of RPTPb mRNA, whereas 1.2% Me 2 WebHL60, the human promyelocytic leukemia cell line, consists of cells that resemble promyelocytes but are transformed into more mature myeloid forms (polymorphonuclear …
Promyelocytic HL60 Cells Express NADPH Oxidase and Are …
WebJan 12, 2024 · HL-60 cells were differentiated with 1.3% v/v DMSO for 6 days with antibiotics or 5 μM of ATRA for 4 days without media change. For nuclei staining HL-60 neutrophil-like cells were treated with 0 ... http://www.plumbingpros.com/index.html gangwar caste category
Histone H3 citrullination as a measure of PAD4 …
WebJan 3, 2014 · First, to estimate whether HL60 acts as a surrogate neutrophils in NET formation process, we treated both primary human neutrophils and neutrophil-like differentiated HL60 cells with PMA, and 1) the amount of extracellularly released histone H3 and 2) chromatin decondensation process were compared between both cells. WebHL-60 and THP-1 cells were differentiated for 72 hours at 37°C with either 25 nM PMA (Item No. 10008014), 1.25% DMSO, 100 mM DMF, 0.1 μM all-trans retinoic acid (ATRA; Item No. 11017), or 100 ... for expression of PAD proteins. For this purpose, HL-60 and THP-1 cells were differentiated with either PMA, DMSO, DMF, ATRA, or vit D3 (Figure … WebPMA-induced NET formation occured sequentially in both neutrophil-like differentiated HL60 cells and primary neutrophils, and Rab27a-knockdown clearly inhibited NET … gangwar er dr institute lucknow