WebEach biopsy sample was divided into two equal parts, one half was fixed in 4% (v/v) buffered neutral formalin and then accordingly embedded in paraffin. Sections were stained by three different methods: H and E staining for histopathological examination, ZN staining, and FF staining for detection of acid-fast bacilli (AFB). WebMoreover, a Fite-Faraco stain showed numerous acid-fast bacilli . A diagnosis of multibacillary leprosy as defined by the World Health Organization (WHO) was made. The patients, without a clear history of exposure to infectious diseases, were subsequently treated with multidrug therapy, consisting of monthly doses of rifampicin 450 mg and ...
Stains for the Microbial Organisms SpringerLink
WebAcid fast stains are used to differentiate acid fast organisms such mycobacteria. Acid fast bacteria have a high content of mycolic acids in their cell walls. Acid fast bacteria will be … WebFive cases of Rhodococcus equi infection in dogs were identified from 2003 to 2014. Three of the dogs had severe, internal lesions attributable to R. equi that have not been previously described: endophthalmitis, endocarditis, and suppurative hill\u0027s onc care
Stains - Libre Pathology
WebNewcomer Supply AFB, Fite Stain Kit procedure is used to detect the presence of either Nocardia sp. or Mycobacterium leprae sp. (causative agent of leprosy) in tissue sections. Procedural variations are included … WebJan 4, 2014 · Discussion. A distinctive feature of bacteria in the genus Mycobacterium is a high content of mycolic acid within the cell wall. The presence of mycolic acid confers to mycobacteria the property of retaining red carbol fuchsin, as in the Ziehl-Neelsen technique (the most commonly used AFS), despite a decolorization procedure with alcohol and … WebDeparaffinize slides in Solution A: Xylene/Peanut Oil, 2:1, two changes for 10 minutes each. Drain slides, wipe off excess oil, and blot to opacity taking care to remove residual oil. See Procedure Note #2. Stain in freshly … smart camping ideas