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Smad3 western blot

WebbBackground The exploration of molecular mechanices underlying transcriptional regulation, particularly in breeding stem cells, has received climb care also concerns the systematic identification of target genes and aforementioned analysis of promoter co-occupancy. High-throughput approaches based on chromatin immunoprecipitation (ChIP) have been … Webbcells and Th17 cells. The specificity of the O72-670 mAb was confirmed by Western blot and immunohistochemistry using unconjugated antibody. The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm.

SMAD3 Monoclonal Antibody (5G11)

Webb11 sep. 2024 · (D) Western blot analysis for Smad2, Smad3, and EGFP from 500,000 cells isolated from hearts of the 2 genotypes of mice shown. (E) Western blot for Tgfbr2 in … Webb13 apr. 2024 · SM reduced relative protein and gene expression in mice peritoneal fibrosis (PF) by RT-qPCR and Western blotting respectively ( n = 6/group). a – d the mRNA expression of TGF-β1, Smad2, Smad3, Smad4, and smad7 in the peritoneal tissue. e Protein bands of TGF-β1, p-Smad2, Smad2, p-Smad3, Smad3, Smad4, Smad7, and GAPDH. f – l … the power station album https://triplebengineering.com

Effects of puerarin on expression of cardiac Smad3 and Smad7 …

Webb3 feb. 2024 · Smad2/3 Antibody (A-3) is a mouse monoclonal IgG 2b κ, cited in 13 publications, provided at 200 µg/ml specific for an epitope mapping between amino … Webb13 apr. 2024 · As the TGF-β/Smad pathway is essential for the development of organ fibrosis, our analysis by RT-qPCR and Western blotting experiments showed that Smad2, … Webb7 apr. 2024 · i Western blot analysis of Flag-NP, p-Smad3, Ripk3, p-MLKL and MLKL expression in the kidneys of 16-week-old db/m and db/db mice with or without … the power starz

Berberine Down-Regulated Myostatin Expression and Facilitated ...

Category:低剂量紫杉醇抑制成纤维细胞增殖、迁移及硬膜外纤维化研究

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Smad3 western blot

SARS-CoV-2 N protein induces acute kidney injury in diabetic mice …

Webb28 juli 2005 · Normal fibroblasts (NF) and keloid fibroblasts (KF) were co-cultured in serum-free medium with normal keratinocytes (NK) or keloid keratinocytes (KK) for 5 days, after … WebbSignaling by transforming growth factor-β (TGF-β) is critical for various developmental processes and culminates in the activation of the transcription factors Smad2 and Smad3. MAN1, an integral protein of the inner nuclear membrane, inhibits TGF-β signaling by binding to Smad2 and Smad3.

Smad3 western blot

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WebbWestern blot analysis of Smad3 in 293T,Jurkat,Rat Heart,Mouse Heart lysates using Smad3 (4H4) antibody. Immunohistochemistry analysis of paraffinembedded Human Liver Tissue using Smad3 antibody. Immunohistochemistry analysis of paraffinembedded Human Colon Carcinoma using Smad3 antibody. 规格: 50μl, 100μl 价格: ¥1400/50μl, … WebbSMAD3 Antibody has been tested for use in ELISA and by western blot. This antibody is suitable in immunohistochemistry. Specific conditions for reactivity should be optimized …

Webb10 juli 2012 · The expressions of TGF-β1/Smad signaling-related genes including type I and III collagen in human and rat HSCs before and after 18α-GA treatment were measured by real time PCR. The expression of related proteins was verified by western blot assay. The phosphorylation level of Smad2 and Smad3 was detected by immunocytochemistry. Webb使用倒置显微镜观察细胞形态,划痕和Transwell实验检测细胞迁移,流式细胞仪分析细胞周期百分比及凋亡率;蛋白印迹法(Western blot)检测各组Smad3、磷酸化Smad3(p-Smad3)、Ⅰ型胶原蛋白(COL1A1)、α-平滑肌肌动蛋白(α-SMA)蛋白表达水平;使用随机数字法将36只成年雄性SD大鼠分为假手术组、对照组和PTX组,每 ...

http://yuxiqbs.cqvip.com/Qikan/Article/Detail?id=7108836075 WebbA human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated …

WebbBiorbyt's SMAD3 antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, and Western Blot. The SMAD3 antibody was generated using SMAD3 as the antigen and it reacts with Human. Description Rabbit polyclonal antibody against SMAD3 conjugated to RPE

WebbWestern blot是评估蛋白磷酸化状态的最常用方法,利用SDS-PAGE分离蛋白样品,随后转移到印迹膜上,之后利用磷酸化特异性抗体来鉴定目的蛋白。. 有人会说western blot还不 … sifilis brainlyWebbWestern blotting (Figure 8D through 8J, p < .05) demonstrated knock-down SAA1 decreased the p-P65, p-IκBα, TGF-β, p-Smad2, and p-Smad3 protein expression, increased IκBα protein levels. The above results indicate that SAA1 is closely involved in the inflammatory response in cardiac fibrosis in vitro. sifilis anticorposWebbAntibodies used to identify and locate intracellular and extracellular proteins in common applications such as Western Blot, ELISA, ImmunoChemistry and Flow Cytometry are all available for your research. Skip to search; Skip to ... Anti-SMAD3 Rat Monoclonal Antibody [clone: 378611] Supplier: R & D Systems. MAB4038. 220020-721EA 600.15 USD ... the power station commandoWebb30 dec. 2016 · Smad3 Antibody (38-Q) is an IgG2a κ mouse monoclonal Smad3 antibody suitable for the detection of Smad3 of mouse, rat and human origin by WB, IP, IF, IHC (P) … the power station communicationWebbHuman Phospho-Smad2/3 (S465/S467) Antibody Summary Species Reactivity Human Specificity Detects human Smad2 and Smad3 when dually phosphorylated at S465 and … the power station discographyWebb摘要: 目的 探讨血管紧张素转化酶2 (ACE2)对缺氧复氧诱导的肾小管上皮细胞HK-2氧化应激、炎症、凋亡及核因子E2相关因子2 (Nrf2)/血红素加氧酶1 (HO-1)信号通路的影响。. 方法 将ACE2慢病毒转染HK-2细胞,按照实验需要分为常氧组 (Control组)、缺氧复氧模型组 (H/R组 … the power station band membersWebbFigure 1 The expression level of miR-21-5p was increased in rats with OVA-induced asthma. Histological changes were analyzed by H&E staining assay (A) (Scale bar = 50 μm) and Masson staining assay (B) (Scale bar = 50 μm).The levels of epithelial cell adhesion factor and stromal cell markers were analyzed by Western blot (C and D, n=6).The … the power station norfolk ne