Tsingke tse005 2×t5 super pcr mix colony

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August undefined, 2024

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Colony PCR SpringerLink

WebApr 13, 2024 · The 1.1×T3 Super PCR Mix (TsingKe, Nanjing, China) was used for PCR. The 10% vertical polyacrylamide electrophoresis was performed to observe the cDNA PCR products. AceQ qPCR SYBR Green Master Mix (Vazyme) was used for qRT-PCR, and GAPDH was used to normalize the level of PD-L1-lnc and PD-L1 mRNA. Primers were listed in … Webmy method: 50 ul h20, pic with a yellow tip a E. coli colony, mix with the water, them heat in the PCR machine at 98ºC, for 10 min, them leave at 4ºc for 5 min. Centrifuge 13000g /10 min, use 2 ... green silk pillowcase australia

2×TransTaq® High Fidelity (HiFi) PCR SuperMix I/II - TransGen …

Web2) Pick 5 transformant colonies. 3) Grow each colony in respective antibiotic containing LB broth for 5-6 hours. 4) Took 500ul of culture and centrifuge. 5) Resuspend in 50ul of nuclease free ... WebMCLAB Products. I-5™ 2X Hi-Fi PCR Master Mix. I-5 High-Fidelity DNA Polymerase is an ultra-high fidelity and high processivity enzyme. It produces the most accurate copies of … WebUltra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes. Indian J Microbiol. 2024 Sep;57 (3):365-369. doi: 10.1007/s12088-017-0665-1. Epub 2024 Aug 10. fms warranty

Creation of new germplasm resources, development of SSR …

http://cdn.casmart.com.cn/Web/IMG/Instructions/2024/04/19-1/174315597-8.pdf Web2×T5 Super PCR Mix（Colony）菌P专家，极速延伸（5~10s/kb）. 【产品特点】. 超快的延伸速度：快速扩增型T5 DNA聚合酶，延伸速度达5 ~ 10 s/ kb；. 极强的耐热性：98 ℃热 … green silk paisley scarfWeb2 O PCR primers OneTaq Master Mix 25 µl PCR Primer 200 nM H 2 O to 50 µl Note: If OneTaq Hot Start Quick-Load 2X Master Mix is used, reactions can be set up at room … green silk pleated dress

"WebFeatures of Platinum SuperFi II PCR Master Mix include: • Exceptional >300X Taq fidelity. • Universal primer annealing at 60°C. • Superior specificity, sensitivity, and yields. • Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement) " - Tsingke tse005 2×t5 super pcr mix colony

Tsingke tse005 2×t5 super pcr mix colony

Fine mapping and target gene identification of - Frontiers

WebPublish time 2024-09-12 17:24. TOROGreen® Yeast colony PCR Kit is used to amplify fragments from the yeast genome as well as transformed plasmids. The same as E … Web2. Dispense PCR master mix (20 µL) into each PCR tube or plate. 3. Using a sterile micropipette tip or a sterile toothpick transfer cells from each colony to a PCR tube and …

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WebJun 9, 2024 · Tsingke Biotech leads the way in the development & manufacturing of genomic and biosynthesis products for global researchers. ... 2×T5 Supper PCR … WebNov 17, 2024 · The amplified product was ligated to the vector according to the requirements of the pClone007 Blunt Vector Kit (TSINGKE, Nanjing, China) and transferred to competent Escherichia coli cells; the potential positive bacteria were cultured at 37 °C and 250 rpm for 6–8 h on a shaker with 2 × T5 Super PCR Mix (Colony) (TSINGKE, Nanjing, …

WebDec 17, 2013 · For example, between 0.05 × 10 5 and 1 × 10 5 cells of Chlorella zofingiensis and between 0.04 × 10 5 and 2.7 × 10 5 cells of Desmodesmus sp. are suggested for use … WebJan 1, 2013 · A single E.coli colony can be used for simultaneous PCR amplification and overnight growth. First, disperse some of the scraped colony into the PCR master mix. Disperse the remainder into a tube containing 1–2 ml of LB medium plus the appropriate antibiotic and grow overnight at 37 °C with shaking.

WebTSINGKE TSE005 2×T5 Super PCR Mix (Colony) 销售价: ￥395.00. 市场价: ￥395.00. 编号: 1216162706837. 货号: TSE005. WebThe SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2 O. • TransTaq ® High Fidelity (HiFi) PCR SuperMix offers 18 …

WebMar 5, 2024 · Then, colony PCR was used to identify the bacterial strain. Briefly, about 2 μL of bacteria were picked into a 50 μL 2 × T5 Super PCR Mix (Colony) system (TSINGKE Biological Technology, Beijing, China) and then amplified and sequenced using primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTA CGACTT-3′).

WebJul 18, 2024 · Polymerase Chain Reaction (PCR) was performed in 12 μl reaction volumes containing 2 template DNA, 5 μl of 2 × T5 Super PCR Mix (PAGE; TSINGKE, Beijing, China), 1 μl of 10 μmol/μL primer pairs, and 4 μl of double distilled H 2 O. Target DNA segments were amplified with the following program, 95°C for 5 min, followed by 35 cycles of 95°C for 10 … green silk oversized shirtWebqRT-PCR Analysis For qRT-PCR analysis, we extracted RNA using the RNAprep Pure Plant Kit (TIANGEN, Beijing, ... The 2 × T5 Super PCR Mix (Colony) was used. ... fmsw6408Webtsingke-shop.oss-cn-beijing.aliyuncs.com green silk shirts for womenWeb4 Use 1 µl of reaction for colony PCR (T5 Taq Polymerase) 5 ... Concentration 2XT5 Super PCR Mix 5ul 1x 10uM Forward Primer 0.4ul 0.4uM 10uM Reverse Primer 0.4ul 0.4uM Template DNA 1ul <1ug green silk literary journalWebFeb 18, 2024 · Prime STAR Max DNA polymerase was bought from Takara (Shanghai, China), T5 super PCR Mix (Colony) DNA polymerase, ... Trelidf™ Prestained Protein … green silk nutraceuticalsWebPick one colony and dissolve in 100 μL media 2 . Take 1 μL as DNA sample for PCR 3 . ... Dream Taq PCR Master Mix(2X) 12.5 Forward Primer 0.1-1.uM Reverse primer 0.1-1.uM … fms.wandafilm.comWeb2、请勿使用dUTP和含有尿嘧啶的引物与模板。 3、PCR Mix应避免反复冻融，短期内多次使用可置于2~8℃保存。使用前，Mix解冻后应充分混匀。保存条件-25℃~-15℃保存，保质期2年，干冰运输。本试剂产品仅供科学研究或进一步生产使用，不可用于临床诊断或治疗。 fm swainson